This simplified practical manual focuses on biochemistry. In medical sciences, biochemistry serves as the Nucleus of Modern Medicine, and plays the role of being the foundation to all other subjects related to its fraternity. The biochemical parameters that it conducts help to understand, diagnose, provide treatment, prognosis and also help in the monitoring of diseases in the modern practice of medicine. This practical manual consists of 33 chapters, which include basic instruments used in a biochemistry laboratory, types of reagents or solutions, sample collection, use of preservatives and anticoagulants, tests for detection of carbohydrates, color reactions of proteins, precipitation reactions of proteins, qualitative analysis of lipids, qualitative reactions of urea, normal urine analysis, automation and quality control, analysis of cerebrospinal fluid, estimation of serum creatinine, urinary creatinine and creatinine clearance test, estimation of uric acid, estimation of transaminases, and estimation of serum alkaline phosphatase. The contents of the book are based on the MCI syllabus for the MBBS course. This manual book is useful for the MBBS students to understand the practical importance of biochemistry.